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hot firepol multiplex mix ready to load  (Solis BioDyne)


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    Structured Review

    Solis BioDyne hot firepol multiplex mix ready to load
    Hot Firepol Multiplex Mix Ready To Load, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hot+firepol+multiplex+mix/HOT+FIREPol+MultiPlex+Mix+Ready+To+Load/pm41932981-331-13-20
    Average 94 stars, based on 7 article reviews
    hot firepol multiplex mix ready to load - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001). .. Second Step PCR was done using Nextera XT Index v2 Primers (Illumina) and HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) doing 10 PCR cycles at optimized temperature. .. Second Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001), quantified using PreciseGreen (Lumiprobe, Catalog No. 12010) and equimolarly pooled.

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
    Article Snippet: First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001). .. Second Step PCR was done using Nextera XT Index v2 Primers (Illumina) and HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) doing 10 PCR cycles at optimized temperature. .. Second Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001), quantified using PreciseGreen (Lumiprobe, Catalog No. 12010) and equimolarly pooled.

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
    Article Snippet: DNA was quantified by PreciseGreen (Lumiprobe, Catalog No. 12010). .. PCR primers (all 5’−3’ orientation) for TBC1D23 for HEK293 clonal lines (forward: ATTACCCAGTTGAGTGCATAGG, reverse: AATGCTGTGGAGGGTATAGCAG) for U2OS clonal lines (forward: TGGAAGCAGGAGGTTGTGAT, reverse: TCTAGATGCGGCATGCTCATAA) for AP1M1 for HEK293 clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) and for U2OS clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) + Illumina Tails was done using HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) at an optimized annealing temperature and ideal PCR conditions (20 PCR cycles). .. First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001).

    Article Title: Stable in vitro propagation of Fagus sylvatica using micro-cuttings established from seedlings
    Article Snippet: .. PCRs were conducted using HOT FIREPol Multiplex Mix (SolisBiodyne, Kisker Biotech Steinfurt, Germany) in a 13 μl reaction volume containing 5–10 ng template DNA in Multiplex PCR Master Mix, which included HOT FIREPol DNA Polymerase (SolisBiodyne, Kisker Biotech Steinfurt, Germany), Multiplex buffer, dNTP mix, BSA, and 10 mM MgCl2. ..

    Article Title: Characterization of Emerging Human Dirofilaria repens Infections, Estonia, 2023
    Article Snippet: .. The PCR reactions were carried out in a volume of 20 μL, using HOT FIREPol MultiPlex Mix (Solis BioDyne, Tartu, Estonia), 0.25 μM of each primer and 10–50 ng of template DNA. ..

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: DNA was quantified by PreciseGreen (Lumiprobe, Catalog No. 12010). .. PCR primers (all 5’−3’ orientation) for TBC1D23 for HEK293 clonal lines (forward: ATTACCCAGTTGAGTGCATAGG, reverse: AATGCTGTGGAG GGTATAGCAG) for U2OS clonal lines (forward: TGGAAGCAGG AGGTTGTGAT, reverse: TCTAGATGCGGCATGCTCATAA) for AP1M1 for HEK293 clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) and for U2OS clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGG TATG) + Illumina Tails was done using HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) at an optimized annealing temperature and ideal PCR conditions (20 PCR cycles). .. First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001).

    Multiplex Assay:

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001). .. Second Step PCR was done using Nextera XT Index v2 Primers (Illumina) and HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) doing 10 PCR cycles at optimized temperature. .. Second Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001), quantified using PreciseGreen (Lumiprobe, Catalog No. 12010) and equimolarly pooled.

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
    Article Snippet: First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001). .. Second Step PCR was done using Nextera XT Index v2 Primers (Illumina) and HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) doing 10 PCR cycles at optimized temperature. .. Second Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001), quantified using PreciseGreen (Lumiprobe, Catalog No. 12010) and equimolarly pooled.

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
    Article Snippet: DNA was quantified by PreciseGreen (Lumiprobe, Catalog No. 12010). .. PCR primers (all 5’−3’ orientation) for TBC1D23 for HEK293 clonal lines (forward: ATTACCCAGTTGAGTGCATAGG, reverse: AATGCTGTGGAGGGTATAGCAG) for U2OS clonal lines (forward: TGGAAGCAGGAGGTTGTGAT, reverse: TCTAGATGCGGCATGCTCATAA) for AP1M1 for HEK293 clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) and for U2OS clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) + Illumina Tails was done using HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) at an optimized annealing temperature and ideal PCR conditions (20 PCR cycles). .. First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001).

    Article Title: Stable in vitro propagation of Fagus sylvatica using micro-cuttings established from seedlings
    Article Snippet: .. PCRs were conducted using HOT FIREPol Multiplex Mix (SolisBiodyne, Kisker Biotech Steinfurt, Germany) in a 13 μl reaction volume containing 5–10 ng template DNA in Multiplex PCR Master Mix, which included HOT FIREPol DNA Polymerase (SolisBiodyne, Kisker Biotech Steinfurt, Germany), Multiplex buffer, dNTP mix, BSA, and 10 mM MgCl2. ..

    Article Title: Characterization of Emerging Human Dirofilaria repens Infections, Estonia, 2023
    Article Snippet: .. The PCR reactions were carried out in a volume of 20 μL, using HOT FIREPol MultiPlex Mix (Solis BioDyne, Tartu, Estonia), 0.25 μM of each primer and 10–50 ng of template DNA. ..

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: DNA was quantified by PreciseGreen (Lumiprobe, Catalog No. 12010). .. PCR primers (all 5’−3’ orientation) for TBC1D23 for HEK293 clonal lines (forward: ATTACCCAGTTGAGTGCATAGG, reverse: AATGCTGTGGAG GGTATAGCAG) for U2OS clonal lines (forward: TGGAAGCAGG AGGTTGTGAT, reverse: TCTAGATGCGGCATGCTCATAA) for AP1M1 for HEK293 clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGGTATG) and for U2OS clonal lines (forward: CAGGATCTTCCAGGCAGCAG, reverse: CTGTAGGGTGGCCAGG TATG) + Illumina Tails was done using HOT FIREPol Multiplex Mix (Solis Biodyne, Catalog No. 04-36-00120) at an optimized annealing temperature and ideal PCR conditions (20 PCR cycles). .. First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001).

    other:

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: First Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001).

    Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
    Article Snippet: Second Step PCR products were purified using NGS Clean Beads (CleanNA, Catalog No. CNGS-0001), quantified using PreciseGreen (Lumiprobe, Catalog No. 12010) and equimolarly pooled.



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